منابع مشابه
RAPID-SELEX for RNA Aptamers
Aptamers are high-affinity ligands selected from DNA or RNA libraries via SELEX, a repetitive in vitro process of sequential selection and amplification steps. RNA SELEX is more complicated than DNA SELEX because of the additional transcription and reverse transcription steps. Here, we report a new selection scheme, RAPID-SELEX (RNA Aptamer Isolation via Dual-cycles SELEX), that simplifies this...
متن کاملDevelopment of RNA aptamers as molecular probes for HER2+ breast cancer study using cell-SELEX
Objective(s): Development of molecules that specifically recognize cancer cells is one of the major areas in cancer research. Human epidermal growth factor receptor 2 (HER2) is specifically expressed on the surface of breast cancer cells. HER2 is associated with an aggressive phenotype and poor prognosis. In this study we aimed to isolate RNA aptamers that specifically bind to HER2 overexpressi...
متن کاملdevelopment of rna aptamers as molecular probes for her2+ breast cancer study using cell-selex
objective(s): development of molecules that specifically recognize cancer cells is one of the major areas in cancer research. human epidermal growth factor receptor 2 (her2) is specifically expressed on the surface of breast cancer cells. her2 is associated with an aggressive phenotype and poor prognosis. in this study we aimed to isolate rna aptamers that specifically bind to her2 overexpressi...
متن کاملNon-SELEX selection of aptamers.
Aptamers are typically selected from libraries of random DNA (or RNA) sequences by SELEX, which involves multiple rounds of alternating steps of partitioning and PCR amplification. Here we report, for the first time, non-SELEX selection of aptamers-a process that involves repetitive steps of partitioning with no amplification between them. A highly efficient affinity method, non-equilibrium cap...
متن کاملDevelopment of RNA aptamers as molecular probes for HER2+ breast cancer study using cell-SELEX
OBJECTIVES Development of molecules that specifically recognize cancer cells is one of the major areas in cancer research. Human epidermal growth factor receptor 2 (HER2) is specifically expressed on the surface of breast cancer cells. HER2 is associated with an aggressive phenotype and poor prognosis. In this study we aimed to isolate RNA aptamers that specifically bind to HER2 overexpressing ...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0082667